Journal
/
면역학 및 감염병학
/
伪多蒙多尼亚小殖民地文化及其藻酸盐的定量
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
Please note that all translations are automatically generated. Click here for the English version.

伪多蒙多尼亚小殖民地文化及其藻酸盐的定量

DOI:
10.3791/60466-v

•

09:06 min

•

February 22, 2020

•

, ,
1Department of Biomedical Sciences, Joan C. Edwards School of Medicine,Marshall University, 2Progenesis Technologies LLC,Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine,Marshall University

Chapters

  • 00:04Introduction
  • 00:49SCV Growth Conditions and Physiological Activation of the Salvage Pathway
  • 01:47Uronic Acid Carbazole Assay
  • 04:36ELISA for Alginate Quantitation
  • 06:34Results: Uronic Acid Carbazole Assay, Anti-Alginate mAb-Based ELISA, and Direct Patient Sample Testing
  • 08:15Conclusion

Summary

자동 번역

자동 번역

在这里，我们描述了一个生长条件，以培养伪多蒙多尼亚小殖民地变种。我们还描述了使用传统的尿酸卡巴佐尔测定法和基于藻酸盐的单克隆抗体（mAb）的ELISA检测和定量由P.aeruginosa产生的外糖藻酸盐的两种独立方法。

Tags

Pseudomonas AeruginosaSmall Colony VariantSCVAlginateELISAUronic Acid CarbazolePhysiological ActivationSalvage PathwayOD600

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

共培养模型绿脓杆菌现场人类呼吸道细胞生长的生物膜

铜绿假单胞菌和酿酒酵母流细胞生物膜

RNA的分离绿脓杆菌殖民小鼠胃肠道

长期慢性<em>铜绿假单胞菌</em>在呼吸道感染小鼠

在伴有海藻酸钠生产新基因的鉴定<em>铜绿假单胞菌</em>使用小型<em> himar1</em>水手转座子介导的诱变

绿脓杆菌引起的肺损伤模型

高通量设置的建立筛选小分子能调节C-二GMP信令<em>绿脓杆菌</em

巨噬细胞集落刺激因子分化的人单核细胞衍生的巨噬细胞的培养

复制的有序, 非冗余图书馆的铜绿假单胞菌应变 PA14 座子插入突变体

铜绿假单胞菌感染肺内皮细胞后细胞毒性 Amyloids 的检测方法

Read Article