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1Sheikh Zayed Institute for Pediatric Surgical Innovation,Children’s National Hospital, 2Children’s National Heart Institute,Children’s National Hospital, 3Center for Neuroscience Research,Children’s National Hospital, 4Department of Biomedical Engineering, School of Engineering,University of Alabama at Birmingham, 5Department of Pediatrics, Department of Pharmacology & Physiology, School of Medicine and Health Sciences,George Washington University
Chapters
- 00:05Title
- 01:02Heart Excision and Langendorff Perfusion
- 02:42Electrophysiology Study
- 05:17Transmembrane Voltage and Intracellular Calcium Optical Mapping
- 06:55Cleanup
- 07:20Results: Representative Optical Data from Langendorff-Perfused Model Hearts
- 08:59Conclusion
The objective of this study was to establish a method for investigating cardiac dynamics using a translational animal model. The described experimental approach incorporates dual-emission optocardiography in conjunction with an electrophysiological study to assess electrical activity in an isolated, intact porcine heart model.
