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Developmental Biology

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JoVE Journal Developmental Biology
Laser Capture Mikrodissektion von Maus embryonalen Knorpel und Knochen für Die Genexpressionsanalyse
 
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Laser Capture Mikrodissektion von Maus embryonalen Knorpel und Knochen für Die Genexpressionsanalyse

Article DOI: 10.3791/60503-v • 09:20 min • December 18th, 2019
December 18th, 2019 •
1, 1, 1,2, 1, 1
1Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, 2Icahn Institute for Data Science and Genomic Technology, Icahn School of Medicine at Mount Sinai

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Dieses Protokoll beschreibt lasercapture microdissection for the isolation of knorpel and bone from fresh frozen sections of the mouse embryo. Knorpel und Knochen können durch Kresylviolettfärbung schnell visualisiert und präzise gesammelt werden, um hochwertige RNA für die transkriptomische Analyse zu liefern.

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Entwicklungsbiologie Ausgabe 154 Lasercapture Mikrodissektion Meckel-Knorpel Unterkieferknochen Kresylviolett RNA-Isolation RNA-Sequenzierung
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