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Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
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Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

DOI:
10.3791/60507-v

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10:12 min

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December 20, 2019

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, , ,
1Laboratory of Integrative Biology,Institute of Molecular Genetics of the Czech Academy of Sciences

Chapters

  • 00:04Title
  • 00:46Collagen Sandwich Preparation
  • 01:40Liver Harvest
  • 04:04Primary Hepatocyte Isolation
  • 05:073D Collagen Sandwich Primary Hepatocyte Culture
  • 06:11Immunolabeling
  • 07:47Results: Representative Assessment of Cytoskeleton Function in In Vitro Bile Canalicular Formation
  • 09:34Conclusion

Summary

자동 번역

자동 번역

Presented here is a protocol for the isolation of mouse hepatocytes from adult mouse livers using a modified collagenase perfusion technique. Also described is the long-term culture of hepatocytes in a 3D collagen sandwich setting as well as immunolabeling of the cytoskeletal components to study bile canalicular formation and its response to treatment.

Tags

3D Collagen Sandwich CulturePrimary Mouse HepatocytesBile Canalicular FormationCytoskeletonLiver IsolationHepatocyte IsolationIn Vitro StudiesPerfusionHeparinDMEMCollagen IPBSCell Culture

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