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Leukocyte Infiltration of Cremaster Muscle in Mice Assessed by Intravital Microscopy
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Introduction
- 01:08Optional Surgical Trachea and Carotid Artery Preparation
- 03:06Surgical Cremaster Muscle Preparation
- 04:44Intravital Microscopy
- 05:38Results: Representative Post-Capillary Venule Identification, Rolling Leukocyte Recruitment Patterns, and Hemodynamic and Microvascular Parameter Assessment
- 07:21Conclusion
Here, we show how to perform intravital microscopy on post-capillary venules of the mouse cremaster muscle. Commonly applied to different models of inflammation and sepsis, particularly those induced by chemokines and cytokines, we highlight its relevance in the study of muscolopathies involving exaggerated muscular leukocyte infiltration.
