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定量荧光显微镜为基础的单脂体测定，用于检测单个脂质体之间的成分不均匀性
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A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
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定量荧光显微镜为基础的单脂体测定，用于检测单个脂质体之间的成分不均匀性

DOI:
10.3791/60538-v

•

09:12 min

•

December 13, 2019

•

, ,
1Center for Nanomedicine and Theranostics,Technical University of Denmark, 2Center for Intestinal Absorption and Transport of Biopharmaceuticals,Technical University of Denmark, 3Department of Health Technology,Technical University of Denmark

Chapters

  • 00:04Title
  • 00:58Liposome Preparation
  • 02:14Surface Preparation of Imaging Chamber
  • 02:57Liposome Immobilization
  • 03:42Image Acquisition and Data Analysis
  • 05:21Liposome Size Calibration
  • 06:42Results: Liposome Surface Immobilization and Compositional Inhomogeneity
  • 08:05Conclusion

Summary

자동 번역

자동 번역

该协议描述了脂体的制造，以及如何用荧光显微镜将这些脂体固定在表面上，并单独以大规模平行的方式成像。这允许定量种群单脂质体之间的大小和组成不均匀性。

Tags

Single LiposomeLiposome CompositionLiposome InhomogeneityFluorescence MicroscopyLipid LabelingLipid MixingFreeze-thawExtrusionBSA CoatingStreptavidinSingle-molecule Detection

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