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In vitro stimulatie en visualisatie van extracellulaire val afgifte in gedifferentieerde humane monocyte-afgeleide macrofagen
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
DOI:

08:08 min

November 01, 2019

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Chapters

  • 00:05Title
  • 01:35Polarization of HMDM (Human Monocyte-derived Macrophages)
  • 02:36Stimulation of HMDM to Induce MET (Macrophage Extracellular Traps) Release
  • 04:20Visualization of MET in Live Cell Culture
  • 05:46Results: Morphological Changes of Differentially Polarized HMDMs and Release of METs Following Stimulation
  • 07:17Conclusion

Summary

자동 번역

Hier is een protocol voor het opsporen van macrofaag extracellulaire trap (met) productie in levende celcultuur met behulp van microscopie en fluorescentie kleuring. Dit protocol kan verder worden uitgebreid om specifieke MET eiwit markers te onderzoeken door immunofluorescentie kleuring.

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