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Genetics

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JoVE Journal Genetics
Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform
 

Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine (IdU) with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform

Article DOI: 10.3791/60556-v • 08:37 min • October 22nd, 2021
October 22nd, 2021 •
1, 1
1Ohio State Comprehensive Cancer Center, Division of Hematology, The Ohio State University

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This protocol adapts cell cycle measurements for use in a mass cytometry platform. With the multi-parameter capabilities of mass cytometry, direct measurement of iodine incorporation allows identification of cells in S-phase while intracellular cycling markers enable characterization of each cell cycle state in a range of experimental conditions.

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Keywords: Cell Cycle Mass Cytometry 5-Iodo-2'-Deoxyuridine (IdU) Cell Cycle Markers Acute Myeloid Leukemia Stem And Progenitor Cells Cell Growth Cell Proliferation Live/dead Stain Paraformaldehyde Cell Staining Medium DMSO Iridium
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