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海参肠细胞原培养的凋亡诱导与检测

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Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

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海参肠细胞原培养的凋亡诱导与检测

DOI:

10.3791/60557-v

•

07:47 min

•

January 21, 2020

•

Tianming Wang, Xu Chen, Ke Xu, Bing Zhang, Dexiang Huang, Jingwen Yang

¹National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College,Zhejiang Ocean University

Chapters

00:04Title
00:51Intestinal Cell Preparation
02:25Cell Culture
04:17Apoptosis Induction and Detection in A. japonicus Intestinal Cells
06:03Results: Microscopy and Statistical Analysis
07:08Conclusion

Summary

자동 번역

该协议提供了一种易于处理的方法，用于培养海参黄斑狼的肠道细胞，并与来自海洋生物（包括Echinodermata、Mollusca和甲壳类）的各种广泛可用的组织样本兼容。

Tags

Keywords: Primary Cell Culture Sea Cucumber Intestinal Cells Apoptosis Cell Culture Protocol Marine Invertebrate Trypsin Cell Strainer Centrifugation Cell Culture Medium Antibiotics Incubator

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