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使用阵列库的中等通量筛选和基于双 Luciferase 的分录报告器识别转录因子稳压器
JoVE 신문
암 연구학
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JoVE 신문 암 연구학
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
DOI:

11:32 min

March 27, 2020

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Chapters

  • 00:04Introduction
  • 00:39Lentiviral Vector Library Preparation
  • 01:24Packaging of the Arrayed Lentiviral Library
  • 03:35Cell Infection for Screening
  • 04:48Cell Seeding for Transfection of Dual-Luciferase Reporter
  • 06:27Dual-Luciferase Reporter Transfection
  • 07:35Dual-Luciferase Activity Quantification
  • 09:02Results: Representative Arrayed Library Screening
  • 10:47Conclusion

Summary

자동 번역

为了识别新的转录因子调节器,我们开发了一种使用基于双透明酶的转录报告器测定筛选排列的慢病毒或逆转录病毒RNAi库的方法。这种方法提供了一种快速且相对便宜的方法,在单个实验中筛选数百名候选人。

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