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Biochemistry

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JoVE Journal Biochemistry
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
 

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Article DOI: 10.3791/60616-v • 08:56 min • March 8th, 2020
March 8th, 2020 •
1, 2, 1, 1
1Department of Biology, Concordia University, 2Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry, Concordia University

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요약
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We present a protocol using liquid chromatography coupled with tandem mass spectrometry to identify and quantify major cellular lipids in Saccharomyces cerevisiae. The described method for a quantitative assessment of major lipid classes within a yeast cell is versatile, robust, and sensitive.

Tags

Quantitative Analysis Cellular Lipidome Saccharomyces Cerevisiae Liquid Chromatography Tandem Mass Spectrometry Lipid Classes Extraction Method LC-MS Method Isobaric Lipidic Species Isomeric Lipidic Species Chemical Properties Physical Properties Glucose Stock Solution YNB Medium Yeast Culture Overnight Yeast Culture Yeast Cells Centrifuge Tube Nano-pure Water Ammonium Bicarbonate Buffer Ammonium Bicarbonate Solution
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