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Chapters
- 00:05Introduction
- 00:52Plating TUM622 Cells in the Extracellular Matrix for 3D Culturing
- 03:523D Coculturing of TUM622 Cells and CAFs (Cancer Associated Fibroblasts) in the Extracellular Matrix
- 05:39Results: Growth and Morphology
- 06:58Conclusion
An in vitro model system was developed to capture tissue architectural changes during lung squamous carcinoma (LUSC) progression in a 3-dimensional (3D) co-culture with cancer-associated fibroblasts (CAFs). This organoid system provides a unique platform to investigate the roles of diverse tumor cell-intrinsic and extrinsic changes that modulate the tumor phenotype.
