Journal
/
암 연구학
/
Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts
JoVE 신문
암 연구학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

DOI:
10.3791/60646-v

•

10:27 min

•

July 25, 2020

•

,
1Center for Individualized Medicine,Mayo Clinic, 2Department of Molecular Pharmacology and Experimental Therapeutics,Mayo Clinic

Chapters

  • 00:00Introduction
  • 00:59Selection of Therapeutic Targets
  • 03:45Tumor Engraftment and Maintenance
  • 07:15Testing Responses to Genomically Identified Targets in PDX Models
  • 07:46Representative Results
  • 10:02Conclusion

Summary

자동 번역

자동 번역

Here we present a protocol to test the efficacy of targeted therapies selected based on the genomic makeup of a tumor. The protocol describes identification and validation of structural DNA rearrangements, engraftment of patients’ tumors into mice and testing responses to corresponding drugs.

Tags

Keywords: CancerTargeted TherapyStructural DNA AlterationPatient-derived XenograftsWhole Genome SequencingGenomic AlterationDruggable ChangesIn Vivo TreatmentTreatment EfficacyAlgorithmMicrosequencingGene SymbolsPathway AnalysisFisher’s Exact TestDruggable GenesGene Cards

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/60646?article_type=v

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
check_url/kr/60646?article_type=v

Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
check_url/kr/60646?article_type=v

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
check_url/kr/60646?article_type=v

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
check_url/kr/60646?article_type=v

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
check_url/kr/60646?article_type=v

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
check_url/kr/60646?article_type=v

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
check_url/kr/60646?article_type=v

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
check_url/kr/60646?article_type=v

High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids
check_url/kr/60646?article_type=v

Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids

Read Article