JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People’s Hospital,First Affiliated Hospital of Shenzhen University, Health Science Center, 2Institute for Cellular Therapeutics,Allegheny-Singer Research Institute, 3School of Pharmaceutical Sciences,Wenzhou Medical University, 4Xenotransplantation Program/Department of Surgery,University of Alabama at Birmingham, 5Jiangsu Key Laboratory of Xenotransplantation,Nanjing Medical University
Chapters
- 00:04Title
- 01:13Islet Preparation
- 05:39Inguinal Subcutaneous White Adipose Tissue (ISWAT) Islet Transplantation
- 08:13Results: Representative Islet Morphology and Function
- 09:24Conclusion
Neste protocolo, um método de isolamento e transplante de isto de murina no tecido adiposo branco subcutâneo inguinal é descrito. Ishotas singeneicas isoladas são transplantadas em um receptor de murina usando um hidrogel de membrana do porão. O nível de glicemia dos receptores é monitorado, e a análise de histologia dos enxertos de lumiçal é realizada.
