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量化金黄色葡萄球菌对人多态核白细胞的细胞毒性
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
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量化金黄色葡萄球菌对人多态核白细胞的细胞毒性

DOI:
10.3791/60681-v

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12:27 min

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January 03, 2020

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, , , , , , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Chapters

  • 00:00Title
  • 00:44Polymorphonuclear Leukocyte Purification
  • 04:34Cytotoxicity Assay of S. aureus Extracellular Proteins Against Human Polymorphonuclear Leukocytes
  • 06:16S. aureus Cytotoxicity Against Human Polymorhonuclear Leukocytes Following Phagocytosis
  • 08:40Results
  • 11:02Conclusion

Summary

자동 번역

자동 번역

该协议描述了从全人类血液中纯化多态核白细胞的方法，以及两种独特的测定方法，用于量化金黄色葡萄球菌对这些重要的先天免疫细胞的细胞毒性。

Tags

Staphylococcus AureusCytotoxicityPolymorphonuclear LeukocytesPMNsNeutrophilsBi-component LeukotoxinsMembrane IntegrityInnate Immune CellsCentrifugationDensity GradientsHeparinized Whole BloodDextranFicollCell IsolationCell Separation

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