JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Molecular Physiology and Biophysics,Vanderbilt University, 2Agilent Technologies, 3Department of Biological Sciences,Vanderbilt University, 4Department of Veterans Affairs Tennessee Valley, 5Department of Medicine,Vanderbilt University Medical Center, 6Department of Cell and Developmental Biology,Vanderbilt University
Chapters
- 00:05Title
- 01:12Spheroid Microplate Preparation
- 02:03Sensor Cartridge Hydration
- 02:36Sensor Cartridge Preparation
- 03:00Pancreatic Islet Transfer
- 04:19Oxygen Consumption Assay
- 05:06Results: Representative Pancreatic Islet Oxygen Consumption Analyses
- 06:09Conclusion
Este protocolo demuestra la medición precisa y reproducible del consumo de oxígeno en islotes pancreáticos de primates no humanos. Las técnicas de carga de islos y el recubrimiento de la microplaca proporcionan un marco para la medición eficiente de la respiración en otros tipos de esferoides cultivados.
