Encellet opløsning tredimensionel billeddannelse af intakte organoider

Article DOI: 10.3791/60709-v • 10:40 min • June 5th, 2020

June 5th, 2020 •

Ravian L. van Ineveld*^1,2,3, Hendrikus C.R. Ariese*^1,2,3, Ellen J. Wehrens^1,2,3, Johanna F. Dekkers*^1,2,3,4, Anne C. Rios*^1,2,3

¹Princess Máxima Center for Pediatric Oncology, ²Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, ³Cancer Genomics Center (CGC), ⁴Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht

* These authors contributed equally

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Hele 3D-struktur og cellulære indhold af organoider, samt deres fænotopypiske lighed med det oprindelige væv kan fanges ved hjælp af encellede opløsning 3D-billeddannelse protokol beskrevet her. Denne protokol kan anvendes på en bred vifte af organoider varierende i oprindelse, størrelse og form.

Encellet opløsning tredimensionel billeddannelse af intakte organoider

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