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Journal / Developmental Biology / Imágenes fluorescentes de calcio y posterior…
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Developmental Biology

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JoVE Journal Developmental Biology
Imágenes fluorescentes de calcio y posterior hibridación in situ para la caracterización de precursores neuronales en Xenopus laevis
 
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Imágenes fluorescentes de calcio y posterior hibridación in situ para la caracterización de precursores neuronales en Xenopus laevis

Article DOI: 10.3791/60726-v • 09:07 min • February 18th, 2020
February 18th, 2020 •
1, 2, 3, 4, 4, 5, 5, 2
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University, 2Department of Biology, College of William and Mary, 3Harvard Medical School, Harvard University, 4Department of Bioengineering, California Institute of Technology, 5Department of Computer Science, College of William and Mary

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Presentamos un protocolo de dos partes que combina imágenes fluorescentes de calcio con hibridación in situ, permitiendo al experimentador correlacionar patrones de actividad de calcio con perfiles de expresión génica a nivel de una sola célula.

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Biología del desarrollo Número 156 Ca2+ calcio imágenes de calcio actividad de calcio desarrollo neuronal desarrollo embrionario neurotransmisor neurociencia
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