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TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences,China Agricultural University, 2Department of Plant Biology, College of Biological Sciences,University of California, Davis, 3Genome Center, College of Biological Sciences,University of California, Davis
Chapters
- 00:04Introduction
- 01:01Agroinfiltration and Leaf Sample Collection
- 01:59Extraction of Leaf Total Protein and Removal of Free Biotin
- 04:11Enrichment of Biotinylated Proteins
- 05:53Results: Western Blot of Protein Extracts
- 06:25Conclusion
Described here is a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in Nicotiana benthamiana leaf tissue. Also provided is a detailed protocol for the identification of interactions between other proteins of interest using this technique in Nicotiana and other plant species.
