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تطوير نموذج عدوى حمار وحشي لـ Clostridioides difficile
JoVE 신문
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JoVE 신문 발생학
Development of a Larval Zebrafish Infection Model for Clostridioides difficile
DOI:

09:13 min

February 14, 2020

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Chapters

  • 00:05Introduction
  • 00:36Preparation and Labeling of C. difficile and Spores with Fluorescent Dye
  • 01:47Injection of Stained C. difficile into Zebrafish Larvae
  • 03:40Gavage of Zebrafish Larvae and Confocal Microscopy Analysis
  • 05:28Dissection of Larval Zebrafish Intestine to Recover Viable C. difficile
  • 06:35Results: Infection of C. difficile in Zebrafish
  • 08:27Conclusion

Summary

자동 번역

عرض هنا هو وسيلة آمنة وفعالة لتصيب يرقات حمار وحشي مع اللاهوائية الفلورية C. difficile عن طريق الحقن المجهري وmicrogavage غير الباضعة.

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