JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Introduction
- 00:55Embryo Staining and Embedding
- 03:03Methanol Dehydration and Tissue Clearing
- 04:04Embryo Mounting
- 04:40Third Pharyngeal Arch (PA) Surfacing
- 05:57Surfaced Structure Masking
- 07:01Endothelial Cell Quantification
- 08:19Results: Representative PA and PAA Surfacing and EC Quantification
- 09:28Conclusion
Tags
VisualizationAnalysisPharyngeal Arch ArteriesWhole-mount Immunohistochemistry3D ReconstructionEndothelial Cell QuantificationAortic Arch ArteriesCongenital Heart DiseaseVessel FormationRemodelingLight-sheet MicroscopyMouse EmbryoFixationPermeabilizationBlocking BufferPrimary AntibodySecondary AntibodyPlastic Paraffin Molds
