Journal
/
생물학
/
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

DOI:
10.3791/60800-v

•

08:18 min

•

June 16, 2020

•

, , , ,
1Advanced ICT Research Institute Kobe,National Institute of Information and Communications Technology, 2Graduate School of Frontier Biosciences,Osaka University, 3Micron Advanced Bioimaging Unit, Department of Biochemistry,University of Oxford

Chapters

  • 00:04Introduction
  • 00:40Sample Preparation for Imaging
  • 02:50Acquisition of Reference Images
  • 04:47Correction of Chromatic Shift Using Chromagnon Software
  • 06:25Results: Chromatic Shift Correction with Chromagnon
  • 07:51Conclusion

Summary

자동 번역

자동 번역

Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software, Chromagnon.

Tags

3D Chromatic ShiftChromatic Aberration CorrectionSuper-resolution MicroscopyBiological ImagingChromagnonColocalization AnalysisWidefield MicroscopyFluorescent StainingPhalloidinAlexa FluorMounting Medium

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/60800?article_type=v

A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila
check_url/kr/60800?article_type=v

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
check_url/kr/60800?article_type=v

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model
check_url/kr/60800?article_type=v

Super-resolution Imaging of the Bacterial Division Machinery
check_url/kr/60800?article_type=v

Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish
check_url/kr/60800?article_type=v

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
check_url/kr/60800?article_type=v

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
check_url/kr/60800?article_type=v

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
check_url/kr/60800?article_type=v

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
check_url/kr/60800?article_type=v

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Read Article