Journal
/
면역학 및 감염병학
/
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

DOI:
10.3791/60806-v

•

07:50 min

•

March 30, 2020

•

, , ,
1Robert Lurie Comprehensive Cancer Center and Department of Dermatology, Feinberg School of Medicine,Northwestern University, 2Department of Pathology,University of Illinois at Chicago, 3Department of Microbiology and Immunology, Feinberg School of Medicine,Northwestern University

Chapters

  • 00:05Introduction
  • 00:39Antibody and Fluorophore Selecting and Staining
  • 01:52Spectral Library Preparation
  • 02:59Multispectral Imaging
  • 03:54Spectral Unmixing
  • 04:37Analyzing Multispectral Images via Cell Segmentation and Phenotyping
  • 05:52Results: Representative Multispectral Imaging and Cell Quantification
  • 07:07Conclusion

Summary

자동 번역

자동 번역

We describe a rapid staining method to perform multispectral imaging on frozen tissues.

Tags

Keywords: Multispectral Fluorescence ImagingBiomarker DetectionCo-localizationRapid Staining MethodSpectral LibraryMachine LearningImage Analysis

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/60806?article_type=v

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
check_url/kr/60806?article_type=v

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
check_url/kr/60806?article_type=v

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
check_url/kr/60806?article_type=v

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
check_url/kr/60806?article_type=v

4D Multimodality Imaging of Citrobacter rodentium Infections in Mice
check_url/kr/60806?article_type=v

Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device
check_url/kr/60806?article_type=v

A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood
check_url/kr/60806?article_type=v

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
check_url/kr/60806?article_type=v

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood
check_url/kr/60806?article_type=v

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Read Article