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使用免疫荧光对患有TNBS诱导克罗恩病的小鼠结肠雌激素受体的可视化
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Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence
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使用免疫荧光对患有TNBS诱导克罗恩病的小鼠结肠雌激素受体的可视化

DOI:
10.3791/60813-v

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08:02 min

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March 12, 2020

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, , , ,
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection,University of Lodz, 2Department of Biochemistry, Faculty of Medicine,Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection,University of Lodz

Chapters

  • 00:05Introduction
  • 00:49Colon Sample Preparation
  • 03:12Immunohistochemistry
  • 06:07Results: Estrogen Receptor Localization in the TNBS-induced Crohn s Disease
  • 07:20Conclusion

Summary

자동 번역

자동 번역

该协议提出了完整的经验证的克罗恩病TNBS诱导的鼠模型，以及利用嵌入在石蜡中的正式固定结肠部分的免疫荧光，通过免疫组织化学对雌激素受体进行可视化的方法。

Tags

Estrogen ReceptorImmunofluorescenceTNBS-induced Crohn’s DiseaseMouse ColonParaffin EmbeddingMicrotome SectioningDeparaffinization

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