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Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation
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Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

DOI:
10.3791/60815-v

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08:22 min

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January 22, 2020

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, ,
1Department of Pharmacology,Weill Cornell Medical College

Chapters

  • 00:04Title
  • 00:32Sensor Cartridge and Extracellular Flux Analyzer Calibrant, Concanavalin A (ConA)-Coated Microplate, and Sperm Buffer Preparation
  • 01:52Cartridge Calibration Preparation and Wave Template Generation
  • 03:00Mouse Sperm Isolation
  • 03:42Sensor Cartridge Loading
  • 04:59Sperm Plate Preparation
  • 06:17Results: Representative Changes in Glycolysis and Oxidative Phosphorylation During Mouse Sperm Capacitation
  • 07:42Conclusion

Summary

자동 번역

자동 번역

We describe the application of an extracellular flux analyzer to monitor real-time changes in glycolysis and oxidative phosphorylation during mouse sperm capacitation.

Tags

Extracellular Flux AnalyzerGlycolysisOxidative PhosphorylationMouse SpermCapacitationConATYH BufferWave TemplateSperm Metabolism

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