JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Introduction
- 00:54Synthesis of the UAA: Propargyl-L-Lysine (Prk)
- 04:46Production of the Recombinant Protein modified by PrK
- 08:13Removal of the Histidine Tag by TEV Protease Digestion
- 09:48Assessment of PrK Accessibility and Functionality and Conjugation to the Hapten
- 11:26Results: Efficient Incorporation of the PrK and Click Conjugation
- 12:49Conclusion
