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Chapters
- 00:04Introduction
- 00:55C2C12 Transfection
- 02:35Transfected C2C12 Selection
- 03:15C2C12 Differentiation
- 03:59Phenotypic Analysis
- 06:10Western Blot Analysis Sample Preparation
- 09:37Results: Representative C2C12 Selection, Differentiation, and Knockdown Validation
- 11:19Conclusion
We provide a protocol to stably knock down genes encoding extracellular matrix (ECM) proteins in C2C12 myoblasts using small-hairpin (sh) RNA. Targeting ADAMTSL2 as an example, we describe the methods for the validation of the knockdown efficiency on the mRNA, protein, and cellular level during C2C12 myoblast to myotube differentiation.
