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诱导毛茸茸的根由农业细菌根- 在塔特里巴克麦的中介转化 (法戈皮鲁姆塔塔里库姆
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Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)
DOI:

08:12 min

March 11, 2020

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Chapters

  • 00:04Introduction
  • 01:01Tartary Buckwheat (TB) Seed Preparation
  • 01:49TB Seedling and Explant Preparation
  • 02:46A. rhizogenes Transformation Preparation
  • 03:56TB Explant Screening, Infection, and Co-Culture
  • 04:47Induction and Selective Culture and TB Hairy Root Subculture
  • 05:32Transformed Hairy Root Identification and Conservation
  • 06:14Results: Representative Hairy Root Transformation Identification and Characterization
  • 07:23Conclusion

Summary

자동 번역

我们描述了一种通过农业细菌根诱导毛茸茸的根的方法 - 在塔特里麦(法戈皮鲁姆塔塔里库姆)中介导的转化。这可用于研究塔塔麦中继发代谢物的基因功能和产生,用于任何基因转化,或用于改良后的其他药用植物。

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