/
/
Markerless Gene Sletning af Floxed Kassette Allelic Exchange Mutagenesis i Klamydia trachomatis
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Introduction
- 00:27Design and Assembly of pSUmC-4.0 with Homology Arms Specific to the Gene of Interest
- 02:20Transformation of C. trachomatis with pSUmC 4.0 + Homology Arms for Gene Deletion by Allelic Exchange
- 05:54Clonal Isolation of Green-only C. trachomatis Deletion Mutant Containing the loxP Flanked Selection Cassette by Limiting Dilution
- 07:38Transformation of C. trachomatis FRAEM Mutant with pSU-Cre to Initiate Removal of loxP Flanked Selection Cassette
- 08:42Results: FLAEM Enables Markerless Gene Deletion in C. trachomatis and alleviates Cassette-induced Polar Effects
- 09:47Conclusion
