Journal
/
암 연구학
/
Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

DOI:
10.3791/60850-v

•

08:47 min

•

May 01, 2020

•

, ,
1p53 Laboratory, Neuros/Immunos,A*STAR (Agency for Science, Technology and Research)

Chapters

  • 00:04Introduction
  • 01:12Cell Culture and Transient Transfection
  • 03:37Compound Preparation
  • 05:06Luciferase Complementation and Viability Assay
  • 06:30Results: PP242 Disrupts eIF4F Complex Formation
  • 08:04Conclusion

Summary

자동 번역

자동 번역

Here, we present a protocol to measure eIF4E-eIF4G interaction in live cells that would enable the user to evaluate drug induced perturbation of eIF4F complex dynamics in screening formats.

Tags

EIF4F ComplexEIF4E-eIF4G InteractionProtein-protein Interaction AssayLive Cell MonitoringTranslation RegulationCancer ProliferationPPI InhibitorsHEK 293 CellsTransfectionTrypsinization96-well Plate Seeding

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

A Spheroid Killing Assay by CAR T Cells

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

In Vitro Assay to Study Tumor-macrophage Interaction

Monitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells

Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Read Article