Journal
/
/
切り替え可能なプロトポルフィリンIX依存性活性酸素種のin situ光生成によるマウス皮膚における幹細胞ニッチの刺激と組織再生
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
DOI:

10:05 min

May 08, 2020

, , , ,

Chapters

  • 00:04Introduction
  • 01:26Hair Growth and Burn Induction and Long‐Term 5‐bromo‐2 -deoxyuridine (BrdU) Label Retaining Cell (LRC) Generation
  • 02:58Transient Nonlethal Reactive Oxygen Species (ROS) Production
  • 04:37BrdU Detection in Mouse Tail Skin
  • 05:38ROS Detection in Mouse Tail Skin
  • 07:16Results: Effects of Photodynamic mALA Treatment and Transient ROS Production
  • 09:00Conclusion

Summary

자동 번역

このプロトコルの目的は、マウスの皮膚で非致死レベルの活性酸素種(ROS)の一時的なin vivo産生を誘導し、組織内の生理学的応答をさらに促進することです。

Related Videos

Read Article