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在全融合微流体平台中增强患者衍生异种移植物的外体 3D 水凝胶培养物的生存能力
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JoVE 신문 암 연구학
Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform
DOI:

12:12 min

December 05, 2020

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Chapters

  • 00:04Introduction
  • 01:09PDX (Patient-Derived Xenografts) Dissociation and Initial Purification of Stromal Component
  • 03:17Density Gradient Centrifugation-Based Separation of PDX-Derived Clusters from Single Cells
  • 06:03Hydrogel Preparation and Microfluidic Plate Seeding
  • 10:12Results: Cell Staining and Viability Assessment
  • 11:16Conclusion

Summary

자동 번역

该协议演示了启用患者衍生异种移植 (PDX) 的扩展体外培养的方法。通过直接去除不可行的单细胞,一步提高三维水凝胶中多细胞聚类培养的整体生存能力。辅助步骤演示了在全融合微流体平台中 PDX 培养的最佳实践。

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