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成人心脏纤维细胞和肌纤维细胞的分离与表征
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JoVE 신문 생물학
Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
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成人心脏纤维细胞和肌纤维细胞的分离与表征

DOI:
10.3791/60909-v

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10:45 min

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March 12, 2020

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, , ,
1Department of Pathology, Microbiology, and Immunology,Vanderbilt University Medical Center, 2American Red Cross, National Headquarters

Chapters

  • 00:05Introduction
  • 00:52Heart Dissection
  • 01:56Enzymatic Dissociation of the Heart
  • 04:42Fibroblast Isolation by Differential Plating
  • 06:00Isolation of Fibroblasts Using Magnetic Beads
  • 08:24Results: Fibroblasts in Injured and Uninjured Mouse Hearts
  • 09:57Conclusion

Summary

자동 번역

자동 번역

获得成纤维细胞的纯种群对于研究其在伤口修复和纤维化中的作用至关重要。这里描述的是一种详细的方法，从未受伤和受伤的小鼠心脏中分离成纤维细胞和肌纤维细胞，然后通过免疫荧光、RTPCR、荧光辅助细胞分拣和胶原蛋白凝胶收缩。

Tags

Cardiac FibroblastsMyofibroblastsIsolationCharacterizationFlow CytometryBead-based IsolationCollagenase DigestionCell Culture

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