JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Institute of Molecular Cardiology, Department of Medicine,University of Louisville, 2Department of Bioengineering,University of Louisville, 3Diabetes and Obesity Center, Department of Medicine,University of Louisville, 4Department of Pharmacology and Toxicology,University of Louisville, 5Institute of Cardiovascular Sciences,University of Manchester, 6Faculty of Pharmacy,Zagazig University
Chapters
- 00:05Introduction
- 00:49Pig Heart Tissue Slicing
- 02:35Slice Position Adjustment
- 03:27Slice Collection
- 04:03Heart Slice Culture
- 06:19Results: Pig Heart Slice Culture Viability and Functionality Validation
- 07:06Conclusion
Este protocolo describe cómo cortar y cultivar el tejido cardíaco en condiciones fisiológicas durante 6 días. Este sistema de cultivo podría utilizarse como plataforma para probar la eficacia de nuevas terapias de insuficiencia cardíaca, así como pruebas fiables de cardiotoxicidad aguda en un modelo de corazón 3D.
