Journal
/
발생학
/
Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

DOI:
10.3791/60919-v

•

09:41 min

•

May 14, 2020

•

, ,
1Department of Plant and Microbial Biology,University of Zurich, 2Biophore – UNIL, 3Laboratory of Cell and Molecular Biology, Institute of Biology,University of Neuchâtel

Chapters

  • 00:04Introduction
  • 01:08Propagation and Sterilization
  • 01:36Plating
  • 02:20Tissue Treatment (Optional)
  • 02:51Harvesting
  • 03:39Tissue Grinding and Homogenization
  • 04:39Total RNA Sample Collection, Crude Extract Clearing, Bead Washing
  • 05:34Immunoprecipitation
  • 06:49Lab Supply Reconditioning
  • 07:38Results: Representative Cell Type-Specific GFP Expression and Translational Ribosome Affinity Purification (TRAP)/Polysome RNA Quality Assessment
  • 08:47Conclusion

Summary

자동 번역

자동 번역

Translating ribosome affinity purification (TRAP) offers the possibility to dissect developmental programs with minimal processing of organs and tissues. The protocol yields high-quality RNA from cells targeted with a green fluorescent protein (GFP)-labeled ribosomal subunit. Downstream analysis tools, such as qRT-PCR or RNA-seq, reveal tissue and cell type-specific expression profiles.

Tags

Keywords: Ribosome Affinity Purification (TRAP)Arabidopsis ThalianaRoot DevelopmentCell Type-specificPolysome RNALow-input RNALateral Root FormationCell-cell CommunicationCell WallMechanical SignalingSeed SterilizationExogenous TreatmentNAA

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/60919?article_type=v

Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
check_url/kr/60919?article_type=v

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
check_url/kr/60919?article_type=v

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
check_url/kr/60919?article_type=v

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
check_url/kr/60919?article_type=v

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
check_url/kr/60919?article_type=v

Building Finite Element Models to Investigate Zebrafish Jaw Biomechanics
check_url/kr/60919?article_type=v

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
check_url/kr/60919?article_type=v

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
check_url/kr/60919?article_type=v

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
check_url/kr/60919?article_type=v

Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo

Read Article