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Biochemistry

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JoVE Journal Biochemistry
Conventionele BODIPY Conjugates voor Live-Cell Super-Resolution Microscopie en Single-Molecule Tracking
 
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Conventionele BODIPY Conjugates voor Live-Cell Super-Resolution Microscopie en Single-Molecule Tracking

Article DOI: 10.3791/60950-v • 07:49 min • June 8th, 2020
June 8th, 2020 •
1, *1, *1,2, 1
1School of Physics and Astronomy, University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), 2Middlebury College
* These authors contributed equally

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Conventionele BODIPY conjugaten kunnen worden gebruikt voor live-cel single-molecule lokalisatie microscopie (SMLM) door de exploitatie van hun tijdelijk vormende, rood verschoven grond staat dimers. We presenteren een geoptimaliseerd SMLM-protocol om subcellulaire neutrale lipiden en vetzuren in levende zoogdier- en gistcellen op nanoscopische lengteschaal te volgen en op te lossen.

Tags

Biochemie Fluorescentie microscopie Super-resolutie microscopie single-molecule tracking single-molecule lokalisatie microscopie BODIPY grond-staat dimers gist zoogdiercellen
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