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Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay
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Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay

Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay

DOI:
10.3791/60983-v

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05:20 min

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June 11, 2020

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, ,
1Department of Biology,University of Maryland Baltimore County

Chapters

  • 00:04Introduction
  • 00:36Injection of Flies
  • 02:03Dorsal Vessel Dissection
  • 03:07Fixation, Mounting, and Processing
  • 03:50Results: Imaging and Quantification of Phagocytic Events
  • 04:36Conclusion

Summary

자동 번역

자동 번역

This protocol describes an in vivo assay of phagocytosis used to assess and quantify the ability of young and aged Drosophila melanogaster hemocytes to phagocytose bacteria.

Tags

Phagocytic AbilityHemocytesDrosophila MelanogasterIn Vivo Phagocytosis AssayPhagocytic EventsDissectionGlass NeedlesInjectionFluoro ParticlesPH-sensitive ParticlesStereomicroscopeInsect PinsDissection MediaCuticle ScissorsForcepsDorsal Vessel

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