/
/
Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Introduction
- 00:49Luciferase-Based ToxoplasmaGrowth Assay
- 02:25Chemical Compound Inhibition Efficacy Evaluation
- 04:08CRISPR-Cas9-Based Gene Deletion
- 05:59Toxoplasma Transfection
- 06:51Knockout Parasite Cloning
- 08:06Results: Representative LHVS Inhibition Efficacy Against Intracellular Toxoplasma Growth
- 09:22Conclusion
Presented here is a protocol to evaluate the inhibition efficacy of chemical compounds against in vitro intracellular growth of Toxoplasma gondii using a luciferase-based growth assay. The technique is used to confirm inhibition specificity by genetic deletion of the corresponding target gene. The inhibition of LHVS against TgCPL protease is evaluated as an example.
