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1Center for Orthopedic Research and Translational Sciences, Department of Orthopedics and Rehabilitation,Pennsylvania State College of Medicine, 2Department of Biochemistry and Molecular Biology,Pennsylvania State College of Medicine, 3Department of Pharmacology,Pennsylvania State College of Medicine
Chapters
- 00:04Introduction
- 01:13Histomorphometric Analysis Setup
- 01:59Total, Calcified, and Uncalcified Cartilage Area Measurements
- 02:47Chondrocyte Number and Phenotype Evaluation
- 03:17Tibial Articular Surface Perimeter Measurement
- 03:47Subchondral Bone and Marrow Space Area Measurement
- 04:30Anterior and Posterior Osteophyte Area and Synovial Thickness Measurement
- 05:21Results: Representative Histomorphometric Osteoarthritis (OA) Evaluation in Destabilization of the Medial Meniscus (DMM) and Sham Mice
- 07:06Conclusion
Das aktuelle Protokoll legt eine strenge und reproduzierbare Methode zur Quantifizierung morphologischer Gelenkveränderungen fest, die mit Arthrose einhergehen. Die Anwendung dieses Protokolls kann bei der Überwachung des Krankheitsverlaufs und der Bewertung therapeutischer Interventionen bei Arthrose von Nutzen sein.
