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Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
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Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion

DOI:
10.3791/60997-v

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09:23 min

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April 02, 2020

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, ,
1Children’s Mercy Research Institute, Children’s Mercy Kansas City, 2Department of Pediatrics,University of Missouri Kansas City School of Medicine

Chapters

  • 00:05Introduction
  • 00:36U-118MG Glioblastoma Cell Line Culture and Electroporation
  • 02:13Real-Time Cell Analysis System, Cell Invasion and Migration (CIM) Plate, and Electroporated U-118MG Cells Preparation
  • 04:59Baseline Reading, Cell Seeding, and Cell Impedance Measurement and Visualization
  • 06:43Results: Representative Effects of Crk/CrkL Knockdown on U-118MG Cell Migration
  • 08:48Conclusion

Summary

자동 번역

자동 번역

Cancer is a lethal disease due to its ability to metastasize to different organs. Determining the ability of cancer cells to migrate and invade under various treatment conditions is crucial to assessing therapeutic strategies. This protocol presents a method to assess the real-time metastatic abilities of a glioblastoma cancer cell line. 

Tags

Keywords: Real-time MeasurementCancer Cell MigrationCancer Cell InvasionImpedance-basedU-118MG Cell LineTrypsin-EDTACell CentrifugationElectroporationSiRNAReal-time Cell AnalysisExtracellular Matrix GelCell Invasion AssayLow Serum MediumImpedance Measurement

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