JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital,University of Sydney, 2HTRG – Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery,Heidelberg University Hospital, 3Department of Large Animal Clinical Sciences, College of Veterinary Medicine,Michigan State University
Chapters
- 00:04Introduction
- 00:39Spleen, Contralateral Bone Marrow, Ipsilateral Lymph Nodes, and Synovial Tissue Isolation
- 04:18Tissue Processing: Spleen
- 05:16Tissue Processing: Lymph Node and Bone Marrow
- 06:42Tissue Processing: Synovial Tissue
- 07:57Monocyte and T cell Subset Panel Immunostaining
- 10:28Results: Representative Mouse Osteoarthritis Model Immune Cell Subset Analysis
- 11:45Conclusion
Aquí, describimos un protocolo de citometría de flujo detallado y reproducible para identificar subconjuntos de monocitos/macrofagos y células T utilizando ensayos de tinción extra e intracelular dentro del bazo murino, médula ósea, ganglios linfáticos y tejido sinovial, utilizando un modelo quirúrgico establecido de osteoartritis murina.
