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Nachweis der Protein-S-Acylation mit Acyl-Resin Assisted Capture
JoVE 신문
생화학
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JoVE 신문 생화학
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
DOI:

08:31 min

April 10, 2020

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Chapters

  • 00:04Introduction
  • 01:25Cell Lysate Preparation
  • 02:26Acyl-Resin Assisted Capture (Acyl-RAC): Free Thiol Group Blocking
  • 03:54Acyl-RAC: Hydroxylamine (HAM) Cleavage and S-Acylated Protein Capture
  • 05:52S-Acylated Protein Elution and Detection
  • 06:52Results: Representative S-Acylated Protein Detection
  • 07:43Conclusion

Summary

자동 번역

Acyl-RAC (Acyl-Resin Assisted Capture) ist eine hochempfindliche, zuverlässige und einfach durchzuführende Methode zum Nachweis reversibler Lipidmodifikationen von Cysteinrückständen (S-Acylation) in einer Vielzahl biologischer Proben.

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