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反向转录循环介导的等温放大（RT-LAMP）测定，用于对罗非鱼湖病毒进行特定和快速检测
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
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反向转录循环介导的等温放大（RT-LAMP）测定，用于对罗非鱼湖病毒进行特定和快速检测

DOI:
10.3791/61025-v

•

07:47 min

•

May 18, 2020

•

, , , ,
1Department of Biotechnology, Faculty of Applied Science,King Mongkut’s University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine,Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies,Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS),King Mongkut’s University of Technology North Bangkok (KMUTNB)

Chapters

  • 00:03Introduction
  • 00:59RNA Extraction
  • 02:50Primer Design
  • 05:20Demonstration of RT-Lamp Assay
  • 07:10Results
  • 08:19Conclusion

Summary

자동 번역

자동 번역

与传统RT-PCR技术相比，我们提出了一种RT-LAMP测定方法，用于在相对较短的时间内使用简单的仪器检测罗非鱼中的TiLV。该议定书可能有助于控制TiLVD的流行传播，特别是在发展中国家。

Tags

RT-LAMPReverse Transcription Loop-mediated Isothermal AmplificationTilapia Lake VirusTiLVRapid DetectionRNA ExtractionPrimer DesignPrimerExplorerLAMP PrimersSegment 3AquacultureTilapiaVirus Detection

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