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JoVE Journal
Bioengineering

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Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures
 

Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures

Article DOI: 10.3791/61031-v 10:38 min May 9th, 2020
May 9th, 2020

챕터

요약

Described here is a protocol for the determination of four different tryptophan metabolites generated in the kynurenine pathway (kynurenine, 3-hydroxykynurenine, xanthurenic acid, 3-hydroxyanthranilic acid) in the medium collected from cancer cell cultures analyzed by liquid chromatography coupled with a single quadrupole mass spectrometry.

Tags

Keywords: Kynurenines Liquid Chromatography-mass Spectrometry Cancer Cell Cultures Tryptophan Metabolites Sample Preparation Calibration Charcoal-treated Medium Deproteinization Evaporation Reconstitution
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