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면역학 및 감염병학

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多能干细胞人类巨噬细胞的产生与表征

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Production and Characterization of Human Macrophages from Pluripotent Stem Cells

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多能干细胞人类巨噬细胞的产生与表征

DOI:

10.3791/61038-v

•

08:05 min

•

April 16, 2020

•

Martha Lopez-Yrigoyen², Alisha May, Telma Ventura, Helen Taylor, Antonella Fidanza, Luca Cassetta, Jeffrey W. Pollard, Lesley M. Forrester

¹Centre for Regenerative Medicine, Scottish Centre for Regenerative Medicine,University of Edinburgh, ²Centre for Reproductive Health, The Queen’s Medical Research Institute,University of Edinburgh

Chapters

00:04Introduction
00:51Passaging Human iPSCs
01:46Human iPSC Differentiation to Macrophages
04:33High-Throughput Phagocytosis Assay
05:07Results: Phenotype and Function of iPSC-Derived Macrophages
07:08Conclusion

Summary

자동 번역

该协议描述了人类诱导多能干细胞的巨噬细胞的强健生成，以及其后续表征的方法。细胞表面标记表达、基因表达和功能测定用于评估这些iPSC衍生巨噬细胞的表型和功能。

Tags

Macrophage Pluripotent Stem Cells Cell Therapy Genetic Manipulation Cell Culture Cell Differentiation Embryoid Bodies Cell Passaging Cell Harvesting Media Replenishment

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