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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow cytometrisk analyse af lymfocyt infiltration i centralnervesystemet under eksperimentel autoimmun encephalomyelitis
 
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Flow cytometrisk analyse af lymfocyt infiltration i centralnervesystemet under eksperimentel autoimmun encephalomyelitis

Article DOI: 10.3791/61050-v • 09:01 min • November 17th, 2020
November 17th, 2020 •
1,2, 1, 3, 1, 3
1Translational Medicine Research Center, Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology, Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine

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Dette manuskript præsenterer en protokol til at fremkalde aktiv eksperimentel autoimmun encephalomyelitis (EAE) i mus. En metode til isolering og karakterisering af de infiltrerede lymfocytter i centralnervesystemet (CNS) præsenteres også for at vise, hvordan lymfocytter er involveret i udviklingen af CNS autoimmun sygdom.

Tags

Immunologi og infektion multipel sklerose MS eksperimentel autoimmun encephalomyelitis EAE autoimmun sygdom centralnervesystemet CNS T lymfocyt flowcytometri
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