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Análisis citométrico de flujo de infiltración de linfocitos en el sistema nervioso central durante la encefalomielitis autoinmune experimental
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1Translational Medicine Research Center, Ruijin Hospital North,Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology,Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation,Shanghai Jiao Tong University School of Medicine
Chapters
- 00:04Introduction
- 00:53C57BL/6 Mouse Immunization
- 01:58Single Brain Cell Suspension Preparation
- 04:19Flow Cytometric Analysis
- 07:00Results: Representative Experimental Autoimmune Encephalomyelitis (EAE) Course and Brain Lymphocyte Analyses
- 08:22Conclusion
Este manuscrito presenta un protocolo para inducir la encefalomielitis autoinmune experimental activa (EAE) en ratones. También se presenta un método para el aislamiento y caracterización de los linfocitos infiltrados en el sistema nervioso central (SNC) para mostrar cómo los linfocitos participan en el desarrollo de la enfermedad autoinmune del SNC.
