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In Vitro Microfluidic Disease Model til undersøgelse fuldblod-endotel interaktioner og blodprop Dynamics i realtid
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1Department of Pulmonary Diseases, Amsterdam UMC,VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group,University of Twente, 3Applied Stem Cell Technologies Group,University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC,VU University Medical Center, 5Institute of Physiology,Charité-Universitätsmedizin, 6German Heart Center
Chapters
- 00:04Introduction
- 00:59Primary Human Pulmonary Arterial Endothelial Cell (PAEC) Isolation and Culture
- 02:43Flow Chambers and PAEC Monolayer Preparation
- 04:00Washed Human Whole Blood Preparation
- 04:50Flow System Assembly
- 05:41Washed Whole Blood Perfusion
- 06:44Results: Representative Thrombus Formation Characterization
- 08:29Conclusion
Vi præsenterer en in vitro vaskulær sygdom model til at undersøge fuldblods interaktioner med patient-afledt endotel. Dette system gør det muligt at studere trombogen egenskaber af primære endotelceller under forskellige omstændigheder. Metoden er specielt velegnet til at evaluere in situ trombogenicity og antikoagulationsbehandling under forskellige faser af koagulation.
