/
/
P. aeruginosa Co-cultivo 3D infectado de células epiteliales bronquiales y macrófagos en la interfaz aire-líquido para la evaluación preclínica de los antiinfecciosos
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Drug Delivery,Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy,Saarland University, 3Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research, 4Department of Internal Medicine V – Pulmonology, Allergology, Respiratory Intensive Care Medicine,Saarland University Hospital
Chapters
- 00:04Introduction
- 01:26Growth and Differentiation of Cells in Permeable Support Inserts
- 05:29Infection by P. aeruginosa
- 07:00Measurement of Bacterial Proliferation via Colony-forming Units (CFU)
- 08:00Results: Epithelial-macrophage Co-cultures Infected with P. aeruginosa
- 09:48Conclusion
Describimos un protocolo para un modelo de co-cultivo tridimensional de vías respiratorias infectadas, utilizando CFBE41o- células, macrófagos THP-1, y Pseudomonas aeruginosa, establecido en la interfaz aire-líquido. Este modelo proporciona una nueva plataforma para probar simultáneamente la eficacia de los antibióticos, la función de barrera epitelial y los marcadores inflamatorios.
