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1Radiation Biology Research Center, Institute for Radiological Research,Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences,Chang Gung University, 3Department of Radiation Oncology,Chang Gung Memorial Hospital, 4Department of Laboratory Medicine,Mackay Memorial Hospital
Chapters
- 00:04Introduction
- 00:36Cell Culture and Tumorsphere Formation
- 01:53RNA Isolation
- 03:11RNAseq Profiling and Bioinformatics Analysis
- 03:45Driver Gene Selection
- 04:52Results: Representative Tumorsphere Protein and Gene Expression Analyses
- 06:09Conclusion
Præsenteret her er en protokol til at opdage de overudtrykte driver gener opretholde de etablerede kræft stamceller-lignende celler stammer fra kolorektal HT29 celler. RNAseq med tilgængelig bioinformatik blev udført for at undersøge og screene genekspressionnetværk for at belyse en potentiel mekanisme, der er involveret i overlevelsen af målrettede tumorceller.
